Part:BBa_K2022002:Design
TolR, Periplasmic Domain
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
As the sequence came from E. coli's genome, evolution has likely already made it codon optimised. However, this is only a single domain of the protein, hence start and stop codons had to be artificially added to ensure it is a proper coding sequence. A previous team attempted to submit this part to the registry (BBa_K257005, by Paris 2009), but forgot to add the start codon; we thus submit this part to amend that error.
Another improvement we made was to add a second stop codon at the c-terminus, to more stringently terminate translation.
Source
Derived from the genomic sequence of E. coli K12, but with added N-terminal ATG (to start translation), and C-terminal TAATAA (to stop translation).
References
Henry, T., Pommier, S., Journet, L., Bernadac, A., Gorvel, J.P. and Lloubès, R., 2004. Improved methods for producing outer membrane vesicles in Gram-negative bacteria. Research in Microbiology, 155(6), pp.437-446.